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sp 2 0 myeloma cell line  (ATCC)


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    Structured Review

    ATCC sp 2 0 myeloma cell line
    Sp 2 0 Myeloma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp+2+0+myeloma+cell+line/Sp2%2F0-Ag14/us12599668-2598-19-24
    Average 96 stars, based on 759 article reviews
    sp 2 0 myeloma cell line - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Injection:

    Article Title: Antibodies
    Article Snippet: .. Four days after the last injection, spleen and lymph nodes are harvested, and hybridomas made by standard method using SP 2/0 myeloma cell line (ATCC #CRL-1581) as a fusion partner. ..

    Article Title: High throughput identification of human monoclonal antibodies and heavy-chain-only antibodies to treat snakebite.
    Article Snippet: .. Four days after the last injection, spleen and lymph nodes were harvested, and hybridomas made by the standard method using SP 2/0 myeloma cell line (ATCC#CRL-1581) as a fusion partner as described in detail in Drabek et al. (2022). ..

    Article Title: Antibodies
    Article Snippet: .. Four days after the last injection, spleen and lymph nodes are harvested, and hybridomas made by standard method using SP 2/0 myeloma cell line, (ATCC #CRL-1581) as a fusion partner. ..

    Article Title: A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
    Article Snippet: .. Four days after the last injection, spleen and lymph nodes were harvested, and hybridomas made by standard method using SP 2/0 myeloma cell line (ATCC #CRL-1581) as a fusion partner. ..

    Generated:

    Article Title: An ACE2-blocking antibody confers broad neutralization and protection against Omicron and other SARS-CoV-2 variants
    Article Snippet: .. High-titre mice were euthanized three to five days after the last protein boost (5 in total), B cells were collected from lymphoid tissues (lymph nodes and spleen), and hybridomas were generated by standard method using SP 2/0 myeloma cell line (ATCC #CRL-1581) as a fusion partner. ..

    Cell Culture:

    Article Title: Development of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein for COVID-19 antigen detection.
    Article Snippet: BALB/c mice were immunized with purified recombinant SARS-CoV-2 N protein (50 μL subcutaneously and 100 μL intraperitoneally) with Ribi (sigma) adjuvant after 2 week interval. .. To generate hybridomas, spleen cells were fused with SP 2/0 myeloma cell line (ATCC#CRL-1581) with polyethylene glycol 1500 (PEG 1500) and cultured in HAT-1640 medium containing Hypoxanthine (H), aminopterin (A), and thymine (T). ..

    Article Title: Development of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein for COVID-19 antigen detection
    Article Snippet: BALB/c mice were immunized with purified recombinant SARS-CoV-2 N protein (50 μL subcutaneously and 100 μL intraperitoneally) with Ribi (sigma) adjuvant after 2 week interval. .. To generate hybridomas, spleen cells were fused with SP 2/0 myeloma cell line (ATCC#CRL-1581) with polyethylene glycol 1500 (PEG 1500) and cultured in HAT-1640 medium containing Hypoxanthine (H), aminopterin (A), and thymine (T). ..

    HAT Assay:

    Article Title: Development of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein for COVID-19 antigen detection.
    Article Snippet: BALB/c mice were immunized with purified recombinant SARS-CoV-2 N protein (50 μL subcutaneously and 100 μL intraperitoneally) with Ribi (sigma) adjuvant after 2 week interval. .. To generate hybridomas, spleen cells were fused with SP 2/0 myeloma cell line (ATCC#CRL-1581) with polyethylene glycol 1500 (PEG 1500) and cultured in HAT-1640 medium containing Hypoxanthine (H), aminopterin (A), and thymine (T). ..

    Article Title: Development of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein for COVID-19 antigen detection
    Article Snippet: BALB/c mice were immunized with purified recombinant SARS-CoV-2 N protein (50 μL subcutaneously and 100 μL intraperitoneally) with Ribi (sigma) adjuvant after 2 week interval. .. To generate hybridomas, spleen cells were fused with SP 2/0 myeloma cell line (ATCC#CRL-1581) with polyethylene glycol 1500 (PEG 1500) and cultured in HAT-1640 medium containing Hypoxanthine (H), aminopterin (A), and thymine (T). ..



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